punctatum, the total number of secondary metabolites and active principles thus far described in the species reaches the cipher of two (according to the newest bibliographic review on the chemical content of the Polygonum genus, 2014).ĪBSTRACT Based on a rigorous updated review of the characteristics of the spread of COVID 19, we present for its disclosure our method of personal prevention of viral infection. With the present reporting of compound 1 in P. punctatum for which only polygodial, a potent antifungal sesquiterpene, was previously described. The bibliographic research allowed to label -sitosterol (1) as a new compound in the species P. The spectroscopic work and the structural elucidation permitted the identification of compound 1 as -sitosterol. punctatum and the isolated compound 1 for which a series of reports about its pharmacological properties permitted to affirm that 1, being the major secondary metabolite in the alcoholic extract of roots, is one of the active principles of the plant, namely the anti-inflammatory principle. A bibliographic research allowed to establish a correlation between traditional medicine information of anti-inflammatory property of P. Compound 1 was extracted by maceration in EtOH 96° and it was isolated by using chromatographic techniques. Compound 1 is found to be the major component of the alcoholic extract of roots of Polygonum punctatum (Polygonaceae), a macrophyte native species of Paraguay with varied therapeutic applications like anti-inflammatory among others. The aim of this article is to provide full NMR spectra (1H 500 MHz, 13C 125.8 MHz) of stigmast-5-en-3-ol (1), whose other common names are: -sitosterol, -sitosterin, 22,23-dihydrostigmasterol, and IUPAC name: 17-(5-ethyl-6-methylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1-cyclopentaphenanthren-3-ol., and IUPAC name: The spectra set includes HMBC, HSQC and COSY studies.

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